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Use of Fucoidan Sulfated polysaccharides are previously shown to inhibit virus adsorption or attachment to the surface of animal cells. The sulfates in fucoidan structure were shown to play an important role in the binding of WSSV. It also controls infection by inhibiting replication of enveloped viruses. Thus, it is a potent prophylactic agent against virus infections, WSSV included. Fucoidan is a kind of sulfated polysaccharide obtained from Okinawa-mozuko, Cladosiphon okamuranus a brown sea alga. To test the efficacy of fucoidan in inhibiting WSSV infection in kuruma shrimp, Dr. Itami prepared shrimp diets with different levels of fucoidan and administered it to kuruma shrimp. Fucoidan were fed at concentrations of 10, 20, 60 and 100mg/kg bodyweight/day for 15 days. The control group was fed with 0 mg fucoidan. Four days after the first feeding, the shrimps were immersed in WSSV suspension. Mortality was monitored. Results showed that survival rates of 100 mg fucoidan-fed group was 82.3%, 60 mg group 78.9%, 20 mg group 46.1%, 10mg group 38.4% and control group 12.4%. A dose of fucoidan at60 mg/kg bodyweight/day or higher is effective in preventing WSS in kuruma shrimp. In another experiment, shrimps that were fed intermittently with fucoidan showed a lower survival rate. Shrimps that were fedfucoidan everyday exhibited the highest survival rate. It was concluded that fucoidan should be fed everyday to prevent infection. For details on use of PG and Fucoidan as against WSSV.
contact: Dr. Toshiaki Itami Department of Applied Aquabiology National Fisheries University2-7-1, Nagata-honmachi, Shimonoseki 759-Yamaguchi, Japan Tel: +81 832 86 5111 ext. 466, 459Fax: +81 832 86 7435E-mail: itamit@fish-u.ac.jp |
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